PREPARE OF 5% DEXTROSE SOLUTION

Learn [pharmacology practical experiment]

Preparation of 5% and 10% dextrose solution

THEORY:-

1.Dextrose Monohydrate:-

Dextrose monohydrate (C6H12O6·H2O) is a crystalline powder that is highly soluble in water. It is commonly used as a source of carbohydrates in intravenous fluids and other medical applications.

2.Concentration:-

The concentration of a solution is expressed as a percentage (% w/v) which represents the weight of the solute (dextrose) per 100 mL of solution.

3.Solubility:-

 Dextrose monohydrate is highly soluble in water with a solubility of approximately 60 g/100 mL at 20°C.

4.Solution Preparation:-

To prepare a 5% or 10% dextrose solution, the required amount of dextrose monohydrate is dissolved in distilled water. The solution is then mixed thoroughly to ensure complete dissolution of the dextrose.

APPARATUS:-

                                    1=balance machine

                                    2=beaker

                                    3=stirrer

                                    4=spatula

                                    5=flask

                                    6=funnel

PREPARATION OF 5% DEXTROSE SOLUTION:-

Materials Needed:-

- Dextrose monohydrate (C6H12O6·H2O)

- Distilled water

- Autoclave or sterile filtration equipment

Calculation:-

To prepare 1 liter (1000 mL) of 5% dextrose solution

Preparation Steps:-

1. Weigh 50 g of dextrose monohydrate using a balance.

2. Transfer the weighed dextrose to a sterile container.

3. Add a small amount of distilled water to the container and mix until the dextrose is completely dissolved.

4. Add the remaining distilled water to the container and mix well.

5. Autoclave or sterilize the solution using a 0.2 μm filter.

6. Label and store the solution in a sterile container.

PREPARATION OF 10%:-

To prepare 1 liter (1000 mL) of 10% dextrose solution:

- Weight of dextrose required = 10% of 1000 mL = 100 g

- Volume of distilled water required = 1000 mL

Materials Needed:-

- Dextrose monohydrate (C6H12O6·H2O)

- Distilled water

- Autoclave or sterile filtration equipment

Preparation Steps:-

1. Weigh 100 g of dextrose monohydrate using a balance.

2. Transfer the weighed dextrose to a sterile container.

3. Add a small amount of distilled water to the container and mix until the dextrose is completely dissolved.

4. Add the remaining distilled water to the container and mix well.

5. Autoclave or sterilize the solution using a 0.2 μm filter.

6. Label and store the solution in a sterile container.

STERILIZATION AND STORAGE:-

=STERILIZATION the preparation solution by autoclave or filteration.

=Store the solution in a clean, STORAGE container.

PROCEDURE:-

=Wash all apparatus and dry them.

=Weight all ingredients (e,g 100g and 50g of dextrose sugar.

=Now take some quantity of sterile water in beaker and dextrose add.then stir it until it dissolve completely.

=Add More water if required to make final volume 100ml

IMPORTANT NOTE:-

=Always use distilled or purified water to prepare the solution.

=Follow proper sterilization and storage procedure to maintain the solution quality and safety.

USES OF DEXTROSE:-

1=treatment of hyperglycemia:-

                                                             Dextrose solution are used to rapid increase blood sugar level in individual with hyperglycemia.

2=energy source:-

                                  Dextrose solution provide a quick source of energy for individual who required it. Such as athletic or patient with high energy need.

3=nutrition support:-

                                        Dextrose solution can be used as source of carbohydrates in nutrition support therapy, such as total parental nutrition.

4=medical procedure:-

                                           Dextrose solution may be used as a vehicle for administration medication or as a flushing agent during medical procedure.

5=administration:-

                                    Dextrose solution can be administration orally, intravenously (i.v),or subcutaneous, depending on the specific use and individuals need.

Used in hyperglycemia, insuline shock or dehydration.

Quality Control:-

- Test the solution for sterility using a validated method.

PRECAUTIONS:-

1=allergic reactions:-

                                      Monitor for sign of allergic reactions,such as hives, iteching or difficult breathing.

2=blood sugar level:-

                                       Monitor blood sugar levels close,esp especially in individual with diabetes or hyperglycemia.

3=electrolytes Imbalance:-

                                                  Dextrose solution can be use electrolytes im balance,monitor electrolytes level closely.

4=infection:-

                        Follow proper infection control procedure when administration dextrose solution.